• Edge Color Legend:
  •     ▮ North Tile Edge (1)
        ▮ East Tile Edge (2)
        ▮ South Tile Edge (3)
        ▮ West Tile Edge (4)
  • Instructions:
  • 1) Double click anywhere to make a new tile
    2) Click on a tile to select it and edit its values below (or click and drag a tile to relocate it)
    3) Editable values of a tile:
        Location: x and y
        Rotation: r = 1 to 4
        G/R (giving and receiving identities of 4 edges):
           giving = 1 to 4
           receiving = 0
           inert = #
        E1 to E4 (11 locations of each edge, 5' to 3'):
           _ = scaffold loop
           0 = staple with two stacking bonds
           1 = 1-nt staple
           2 = 2-nt staple
           # = double-hairpin (DHP) staple
    4) Load a FracTile 1x1 Layout to apply a pattern to selected tile
    5) Download sequence information, edge mixes, and tile protocols

    - Example File Competitive Reconfiguration
    - Example File Sequential Reconfiguration
    - Example File Cooperative Reconfiguration

  • Load/Save:
  • Load Full Layout:
  • Load Tile to Focus:
  • Add a Saved Tile: (may be displayed on top of a current tile, click and drag to relocate)
  • Apply Pattern to Selected Tile (FracTile layout 1x1):
  • Load FracTile Layout:
  • Tools:
  • Canvas Size:
  • Edit Selected Tile:
        x: y: r: G/R:
  •     E1:
        E2:
        E3:
        E4:
  •     Final Volume (uL):
    Tile Number:
       
        Tile Name (no commas):



  • Master View (All Object Data):
  • Generate Mixing Protocols:
  • Use 100 uM stock staples to make 50 nM origami with 5x staples
  • See FracTile Compiler for Bridge and Internal Mix details

  • - Unique Edge Staples Required
  • - Edge Staple Mixes for each Tile
  • - Tile Mixes
DTD Designer